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Image Search Results
Journal: Bioconjugate Chemistry
Article Title: Dextran as a Generally Applicable Multivalent Scaffold for Improving Immunoglobulin-Binding Affinities of Peptide and Peptidomimetic Ligands
doi: 10.1021/bc500226j
Figure Lengend Snippet: Figure 6. Size-exclusion chromatography multiangle light-scattering (SEC-MALS) analysis of (a) anti-FLAG mouse IgG1 (green) and FLAG-dextran (purple) and (b) a complex formed in a 1:10 mixture of anti-FLAG mouse IgG1 and FLAG-dextran (black). For each chromatogram, the measured molar mass (left axis) as a function of elution volume is overlaid on the UV signal. In the case of the complex formed between the anti-FLAG IgG and FLAG-dextran (b), the composite molar mass is shown in black, the contribution from the FLAG-dextran is shown in purple, and the contribution from the bound antibody is shown in green. (c) Schematic illustration of an average complex of FLAG-dextran and anti-Flag mouse IgG1.
Article Snippet: The eluent flowed through a UV detector (Agilent), a
Techniques: Size-exclusion Chromatography, Multi-Angle Light Scattering